This detailed volume explores recent advances in phenotypic screening, such as high-resolution microscopy, high-throughput screening, and artificial intelligence (AI)-based image and data analysis tools. The methodologies found within the book enable the characterization of pathomechanisms of human diseases, support the targeted development of novel drugs and personalized medicine, and also aid in the development of novel chemicals to combat human parasites or to identify undesired effects caused by environmental pollutants. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, Phenotypic Screening: Methods and Protocols serves as an ideal guide to this growing field that touches areas of basic and translational biomedical research, clinical and pharmaceutical research, biotechnology, toxicology, and bioinformatics. Chapters 2, 3, 4, 5, 6, 7, 8, and 18 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.